Meridian Bioscience High-Specificity PFU HS Mix - 200 Reactions

A high-fidelity DNA polymerase (higher than 130x Taq fidelity), High-Specificity Pfu HS Mix is highly suited to DNA target enrichment, NGS library amplification, and cloning applications. High-Specificity Pfu HS Mix provides high yield and sensitivity, as well as superior amplification of GC- and AT-rich targets. The hot-start aptamer binds reversibly to the high-fidelity DNA polymerase which inhibits its activity at ambient temperatures and improves the specificity of the amplification.

High-Specificity Pfu HS Mix is a combination of the latest advances in buffer chemistry and PCR enhancers and stabilisers, together with an aptamer-mediated hot-start polymerase, dNTPs, and MgCl₂. The advanced buffer chemistry has been developed for fast, highly reproducible, multiplex PCR and is designed for superior sensitivity and specificity, making High-Specificity Pfu HS Mix perfect for DNA target enrichment and NGS library amplification.

• Aptamer hot start for increased specificity
• Inhibitor-tolerant (tolerates up to 20% whole blood)
• Robust amplification across different GC-content (29% - 65%)
• High-fidelity reduces errors for NGS library amplification